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How do I find the phenotypic ratio?
Finding the phenotypic ratio involves determining the proportion of different observable traits in offspring resulting from a cross between two parents. Here's a step-by-step guide to calculate it:
Using a Punnett Square
- Create a Punnett Square: Draw a grid representing the possible genotypes of offspring. For a simple trait, this is typically a 2x2 square.
- Label Alleles: Write the alleles from each parent along the top and side of the square.
- Fill in Genotypes: Combine alleles from each parent to fill in each square.
- Group Phenotypes: Combine genotypes that result in the same phenotype. For example, both homozygous dominant (AA) and heterozygous (Aa) genotypes may express the dominant trait.
- Count Phenotypes: Count the number of each phenotype group.
- Write the Ratio: Express the counts as a ratio. For instance, if there are three times as many of one phenotype as another, the ratio is 3:1.
Using a Frequency Chart
- Make a Frequency Chart: List the desired traits in columns and count the number of individuals with each trait.
- Rank Frequencies: Arrange the frequencies from smallest to largest.
- Divide by the Smallest Frequency: Divide each frequency by the smallest one to get the ratio components.
- Round and Write the Ratio: Round the results and express them as a ratio (e.g., 9:3:1).
Example
Consider a cross between two heterozygous parents for a trait where "T" is dominant (tall) and "t" is recessive (short). The Punnett Square would show:
- Homozygous Dominant (TT): 1 square
- Heterozygous (Tt): 2 squares
- Homozygous Recessive (tt): 1 square
The genotypic ratio is 1:2:1, but since both TT and Tt express the dominant trait (tall), the phenotypic ratio is 3:1 (tall:short).
Tools
- Punnett Squares: Visual tool for predicting genotypes and phenotypes.
- Phenotypic Ratio Calculators: Online tools that can simplify complex calculations, especially for dihybrid or trihybrid crosses.
By following these steps, you can determine the phenotypic ratio for any given cross using either a Punnett Square or a frequency chart123.